select ad.sno,ad.journal,ad.title,ad.author_names,ad.abstract,ad.abstractlink,j.j_name,vi.* from articles_data ad left join journals j on j.journal=ad.journal left join vol_issues vi on vi.issue_id_en=ad.issue_id where ad.sno_en='99591' and ad.lang_id='5' and j.lang_id='5' and vi.lang_id='5' Detection and Antimicrobial Resistance Profile of E. coli O1 | 99591
Journal de Food : microbiologie, sécurité et hygiène

Journal de Food : microbiologie, sécurité et hygiène
Libre accès

ISSN: 2476-2059

Abstrait

Detection and Antimicrobial Resistance Profile of E. coli O157:H7 from Slaughterhouses and Butcher Shops in Bishoftu Town, Central Oromia, Ethiopia

Fufa Abunna*, Muhabaw Yimana, Hika Waketole, Takele Beyene, Bekele Megersa

Raw beef consumption is an old tradition and a much favored dish by the majority of Ethiopians. However, prevailing unhygienic processing and distribution practices likely contribute to beef contamination leading to foodborne infections. A cross-sectional study was carried out to investigate the occurrence of E. coli O157:H7 and evaluate its antimicrobial resistance profile in slaughterhouses and butcher shops in Bishoftu town, Ethiopia. A total of 352 samples (120 fecal, 92 beef cut, and 140 environmental swabs) were collected. The isolation and identification process was carried out using selective enrichment media, followed by a latex agglutination test. The isolates were tested for their resistance against 13 antimicrobials using the standard disk diffusion method. Out of 352 samples, 14 (3.97%) were found to be positive for E. coli O157:H7 serotype; of which, 28.6% (4/14), 21.4% (3/14), and 50% (7/14) were from fecal, beef, and environmental swab samples respectively. A significant difference in the occurrences of the pathogen was observed among the sources of samples (p<0.05). Results of the antimicrobial susceptibility test revealed high resistance to three commonly used drugs: Tetracycline (100%), erythromycin (92.8%), and ampicillin (64.3%). All of the E. coli O157 isolates were found to be susceptible to azithromycin, cefotaxime, and chloramphenicol. Of the 14 isolates, 12 (85.8%) of them were found to be resistant to three or more classes of antimicrobial agents. E. coli O157:H7 was detected in samples collected from meat and environmental samples implying the health risk of raw and undercooked meat consumption. Results also showed occurrences of multiple antimicrobial-resistant E. coli O157:H7. Therefore, the current study warrants the need of implementing appropriate hygienic measures in slaughterhouses and butcher shops to safeguard public health.

Clause de non-responsabilité: Ce résumé a été traduit à l'aide d'outils d'intelligence artificielle et n'a pas encore été révisé ou vérifié.
Top