Journal de la science cellulaire et de la thérapie
Libre accès

Abstrait

Effet de la L-arginine sur la fonction des mitochondries dans les cellules myocardiques ischémiques-reperfusionnelles chez le lapin

Chen D, Huang L, Jinbo He, Song D, Yingchun Ma, Lina Lin et Wang W

Objective: To investigate the effect of L-arginine (L-Arg) on the function of myocardial mitochondria during myocardia ischemia - reperfusion (IR). Methods: Dividing randomly the 30 rabbits into three groups (n=10): control group (C), myocardia ischemiareperfusion group (IR) and L-Arginine pretreatment group (L-Arg+IR). The relevant parameters, including myocardial mitochondria respiratory function, Ca2+ concentration ([Ca2+]), malondialdehyde (MDA) concentration, superoxide dismutase (SOD) activity, myocardial adenosine triphosphate (ATP), Adenosine diphosphate (ADP), adenosine monophosphate (AMP) content, the total amount of AMP (TAN, TAN=ATP+ADP+AMP) and energy charge (EC, EC=1/2ADP+ATP/TAN) were determined, respectively. Results: The mitochondria respiratory control rate (RCR), III state respiration rate (V3), and SOD in group L-Arg +IR were significantly higher than those in group IR, while IV state respiration rate (V4) , [Ca2+] m, and MDA were obviously lower than those of group IR, the levels of ATP, ADP, TNA and the EC of myocardium showed significantly higher than those in group IR. There were no significant differences in terms of V3, V4, SOD, MDA, AMP and TAN between group L-Arg+IR and group C. Conclusion: It indicated that L-arginine can reduce the level of the oxygen free radicals and partly attenuatecalcium overload to improve the function of myocardial mitochondria during myocardium ischemia reperfusion injury.