Journal du génie chimique et de la technologie des procédés
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Simultaneous determination of adulterants in dietary food supplements using multivariate data analysis after preconcentration with novel nano sorbents and chromatographic measurement

Ensie Hosseini, Jahan B Ghasemi and Maryam Shekarchi

An analytical method for the simultaneous and reliable detection and quantitative determination of three key adulterants in dietary food supplements was developed. Highpressure liquid chromatography (HPLC) assisted by multivariate curve resolution-alternating least square (MCRALS) analysis was used to detect adulterants in real samples after separation and preconcentration using novel mesoporous carbon nanoparticles. Solid phase extraction (SPE) optimization was accomplished by central composite design (CCD). In order to obtain the best results, the MCR-ALS model was compared with the parallel factor analysis 2 (PARAFAC2) model and validated by estimation of linearity, detection limits, and recovery. The detection limits and linear dynamics were calculated as 1.5, 4.27, 4.77 μgml-1 and 1 - 50, 5 - 20, 5 - 20 μgml-1 for caffeine, ephedrine, and fluoxetine, respectively. Mean recovery for determination of caffeine, ephedrine, and fluoxetine using the developed method was reported 101.75, 91.7, and 92.36, respectively. The results showed that to avoid negative health outcomes associated with the inconsiderate consumption of adulterated food supplements, releasing such products should be carefully regulated.