Pédiatrie clinique : libre accès
Libre accès
ISSN: 2572-0775
ISSN: 2572-0775
Yonghong Jiang*, Zhen Xiao, Zhiyan Jiang, Xiaoyang Zhao, Jiajia Lv
Ethno pharmacological relevance: Qingfei Tongluo formular (QT) is a homemade agent for the Mycoplasma Pneumonia (MPP) treatment developed by pediatrics of Shanghai Longhua hospital, which combining with azithromycin has better clinical curative effect. This study aims at exploring the chemical components and clarifying the possible mechanism involved in.
Materials and methods: LC-MS was used for chemical analysis. The mycoplasma pneumoniae infected mice were treated with AZM and QT (46.25 and 92.5 mg/g) for 3 days. Polymorph nuclear neutrophils (PMNs) and monocytes in Bronchoalveolar Lavage Fluid (BALF) were measured by haemacytometer. IL-6, IL-10, IL-1β and IL-13 in BALF were identified by ELISA assay. HE staining was employed for histological examination. TLR4, COX-2 and NF-κB expressions were assessed by western blot.
Results: 10 compounds were identified in QT. After QT treatment, PMNs and monocytes in BALF were reduced and release of inflammatory cytokines was also mediated. HE staining showed that QT effectively arbitrated the inflammation and embolism in lung tissue. QT also blocked the TLR4-NF-κB-COX-2 signaling.
Conclusion: QT showed favorable effect in MPP treatment by diminishing the inflammation symptoms and regulating the TLR4-NF-κB-COX-2 signaling, which could act as a new agent for MPP therapy.