Chimie Analytique Pharmaceutique : Libre Accès
Libre accès

Abstrait

A Recent Validated Synchronous Spectrofluorimetric Method in Comparative to Ratio Derivative One for Determination of Desmopressin Acetate in Dosage Form and Biological Fluids

Khadiga M. Kelani, Ahmed M.Wafaa Nassar, Wael Talaat, Samir Morshedy

In this study green, rapid, specific and highly sensitive methods have been developed and validated for the determination of desmopressin acetate in pharmaceutical formulations and biological fluids (spiked human plasma). Second derivative synchronous spectrofluorimetric method Ratio derivative spectrophotometric method: Sensitivity ranges of (0.25–2.25 μg/ml) and (2–14 ug/ml) and LOD and LOQ values were found to be (0.060-0.183 ug/ml), (0.049-0.162 ug/ml), respectively. The developed methods were validated according to the (ICH) guidelines demonstrating good accuracies and precisions. The results of the developed methods were statistically compared with those obtained by the reported methods without any significant difference. Both methods were applied in quality control laboratories and in routine analysis for analyzed the drug in presence of its acidic degradation products (stability indicating assay and in biological fluids).