Journal des techniques de chromatographie et de séparation
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Simultaneous Monitoring of Multiple Transitions in Mass Spectrometric Analysis Improves Limit of Detection for Low Abundance Substances in Complex Biological Samples

Dmitriy Shin, Eduard Rogatsky and Alexander V Stoyanov

In mass-spectrometry, simultaneous monitoring of several transitions enables increased assay specificity, but also can be used as a tool to improve the lower limit of quantification. In this report, we propose a new approach to low abundant analyte detection in complex samples. The method consists of collecting two and potentially more, different mass spectrometry transitions with further analysis of a product function. For example, C-peptide detection was evaluated with two transitions monitored (1007.7-147.2 and 1007.7-927.8). Further extension of the method is theoretically analyzed and its potential limitations are discussed.