select ad.sno,ad.journal,ad.title,ad.author_names,ad.abstract,ad.abstractlink,j.j_name,vi.* from articles_data ad left join journals j on j.journal=ad.journal left join vol_issues vi on vi.issue_id_en=ad.issue_id where ad.sno_en='34463' and ad.lang_id='5' and j.lang_id='5' and vi.lang_id='5' AQP7 Up-Regulation in the Skeletal Muscles of Mice with Diet | 34463
Journal de la science cellulaire et de la thérapie

Journal de la science cellulaire et de la thérapie
Libre accès

ISSN: 2157-7013

Abstrait

AQP7 Up-Regulation in the Skeletal Muscles of Mice with Diet Induced Obesity

Yoshihiro Wakayama, Satoshi Hirako, Takahiro Jimi and Seiji Shioda

Aquaporin (AQP) 7 and AQP9 are membrane proteins and are the members of aquaglyceroporin which transports glycerol in addition to water molecule. Glycerol is a direct source of glycerol-3 phosphate for the synthesis of triglycerides. We thought that the expression of AQP7 and AQP9 would be altered in the skeletal myofibers in mice with diet-induced obesity (DIO) as compared with that of control chaw-fed mice. RNA and protein levels of AQP7 and AQP9 were studied in the quadriceps femoris muscles of mice with DIO and normal control mice. Real time quantitative RT-PCR analysis showed that mouse AQP7 mRNA levels in skeletal muscles were significantly higher in mice with DIO than in normal control mice (P<0.01); whereas mouse AQP9 mRNA levels were not different between the two groups (P>0.05). Histochemically the myofibers of mice with DIO contained numerous lipid droplets in oil red O stain samples. Immunohistochemical study of DIO mouse muscles showed enhanced expression of AQP7 at the myofiber surface membranes; while AQP9 expression appeared to be similar to that of normal control mice. These findings imply that the up-regulated expression of AQP7 in DIO mouse muscles facilitates the secretion of glycerol from myocytes.

Clause de non-responsabilité: Ce résumé a été traduit à l'aide d'outils d'intelligence artificielle et n'a pas encore été révisé ou vérifié.
Top