Journal de la nutrition et des sciences alimentaires
Libre accès
ISSN: 2155-9600
ISSN: 2155-9600
Adewale M. Esan*, Charles O. Olaiya, Taiwo A. Awolusi, Cynthia N. Ikeji, Bukola V. Aileno-Khuoria, Oluwole Fagbami, Henry Rinde Y. Adeyemi
Increased salinity contributes majorly to the environmental threats, poor growth and productivity of okra plants worldwide. Induced salt-stressed effects on okra plant were mitigated significantly (P< 0.05) with the use of combined treatments of gibberellic acid and Bacillus subtilis. In this study the okra (NHe 47-4 and Clempson genotypes) seeds were pre-soaked with 0.4, 0.5, or 0.6 mM of gibberellic acid, and control (0) in distilled water respectively for 12 h in the dark. The seeds were air-dried at room temperature and germinated in 10 kg of soil treated with 0, 100, or 200 Mm NaCl in polyethene bags. After two weeks of seed germination, three seedlings per pot were inoculated with Bacillus subtilis. Results showed that the effects of gibberellic acid and Bacillus subtilis ameliorated the harmful effects of salinity stress and concomitantly increased the concentrations of minerals (magnesium, potassium, calcium, and phosphorous), proline, soluble sugar and soluble protein of okra plant in all salinity levels. Antioxidant enzymes activity in salt-stressed okra plant were increased especially at 200 mM NaCl in both genotypes with exception of superoxide dismutase (SOD) in Clempson genotype that showed little or no activity relative to salt-treated control groups. The increase in radical-scavenging ability of 2,2-diphenyl-1-picrylhydrazyl (DPPH), as well as total phenolic and flavonoids potentials of salt-stressed okra plant in this study, was associated with increase antioxidant enzymes activity.