Journal des techniques de chromatographie et de séparation
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A Simple HPLC Method for the Separation of Colistimethate Sodium and Colistin Sulphate

Ligai Bai, Zhengyue Ma, Gengliang Yang, Jing Yang and Jia Cheng

In this paper, a simple high performance liquid chromatography (HPLC) method for the determination of colistimethate sodium in the productions of synthesis from colistin E sulphate was established. A HPLC gradient which was: 20% B+80% A changed to 50% B+50% A in 10 min (A was 0.05% TFA aqueous solution and B was acetonitrile) was used and the separation was realized in 8 minutes. Moreover, this method was also used in the separation of colistin sulphate with good resolution. Compared to the methods reported previously, the present method was finished easier in a much shorter time which is 6 min. LC-MS was used to detect colistin sulphate and the result showed that the two compositions were colistin sulphate E1 and E2 as expected. Good separation and reproducibility were obtained.