Journal des méthodes de diagnostic médical
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Three Cases of Brucellosis Which Misidentified with Automated Bacterial Identification System

Şahin R

Brucellosis can hold every organ and tissue, there is no specific clinical finding. In particular, the liver, bone marrow, spleen and lymph nodes as well as the organs of the lymphoreticular system, but more; heart, genito-urinary system organs, central nervous system can hold different organs and tissues, such as joints. Culture, serology, automated identification systems and polymerase chain reaction are used in the diagnosis. n this paper, three case of brucellosis presented which misidentified with an automated bacteria identification system. The first case; 16-year-old female patient, pilonidal cyst, abdominal pain unspecified preliminary diagnoses, second case; 23-year-old female patient, bacterial meningitis, unspecified prediagnosis, third case; 15-year-old female patient: The bacteria were grown in blood and brain fluid samples which taken from patient. Misidentified as Burkholderia gladioli with automated system (Poenix). Then we used to traditional methods (paint, oxidase, serology) for identification. The samples were found to be positive for oxidase, so we contacted to patients’ clinicians for get additional test (Rose Bengal and Standard Tube Agglutination test (STAT). Rose bengal was found as positive, Tube agglutination was found to be 1/1280, 1/640 and 1/2560 positive, respectively. Bacterial identifications were used with konvantional methods (oxidase, serology) and confirmed as Brucella melitensis by external laboratory with Vitec-2. Although proper treatment and eradication studies, brucellosis is still an endemic disease for our country. Brucellosis which misidentified by automated system. B. melitensis is the most common cause of disease in humans. These cases indicated to us deficiency of automated system. t should be update for identification of Brucella spp. and complete with conventional methods.