Journal de pathologie médicale et chirurgicale
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Study of Antimicrobial Activity of Black Cumin Seeds (Nigella sativa L.) Against Salmonella typhi In Vitro

Amalia Tri Utami, Bogi Pratomo and Noorhamdani

Objective: To determine the effectiveness of extracts of black cumin seeds (Nigella sativa L.) as an antimicrobial against Salmonella typhi in vitro. And can determine minimum inhibitory concentration (MIC) and Minimal bactericidal concentration (MBC) from extracts of black cumin seeds (Nigella sativa L.) against Salmonella typhi.
Design: This experimental study used post-test only control group design with four time repetition. Step one was cultivating bacteria in liquid medium with various concentration of extract, that was 40%, 42.5%, 45%, 47.5%, 50% with two control, extract control and bacterial control.
Results: The MIC (Minimal Inhibition Concentration) is 45% concentration of extract. Step two was plating in NAP (Nutrient Agar Plate) medium. The MBC (Minimal Bactericidal Concentration) is 47.5% concentration of extract. The result of experiment was knew there are different average of Salmonella typhi colony from every group. The result experiment was analyzed by One Way Anova Test. The hypothesis test of MBC show significant differentiation, and then was continued with regression test. The conclusion of this study was the addition of extract concentration occurred reduction average of Salmonella typhi colony.