Journal des techniques de chromatographie et de séparation
Libre accès
ISSN: 2157-7064
ISSN: 2157-7064
Keyur B. Ahir, Emanual M. Patelia and Falgun A. Mehta
A simple, precise, rapid, selective, and economic reversed phase high-performance liquid chromatography (RP-HPLC) method has been established for simultaneous analysis of A Phenomenex C18 (250×4.6 mm i.d) chromatographic column equilibrated with mobile phase 0.02 M Potassium dihydrogen o-phosphate/acetonitrile (55/45, v/v) adjusted to pH 6.5 with Triehtylamine (1% v/v) was used. Mobile phase flow rate was maintained at 1 ml/min and effluents were monitored at 278 nm. The sample was injected using a 20 μl fixed loop, and the total run time was 10 min. Experimental conditions such as pH of mobile phase, column saturation time, selection of wavelength, etc. were critically studied and the optimum conditions were selected. The retention time for PCM, DMP and TMD were 3.76 min, 5.18 min and 4.28 min, respectively. The calibration curve for DMP, PCM and TMD was found to be linear in the range of 0.2–1 μg/ml, 6.5–32.5 μg/ml and 0.75–3.75 μg/ml with a correlation coefficient of 0.9998, 0.9976 and 0.9974. The detection limits for PCM, DMP and TMD were 20 ng/ml, 1.06 ng/ml and 2 ng/ml, respectively, while quantitation limits were 60 ng/ml, 3.23 ng/ml and 6 ng/ml, respectively. This HPLC procedure is economic, sensitive, and less time consuming than other chromatographic procedures. It is a user-friendly and importance tool for analysis of combined tablet dosage forms.