Journal of Glycomics & Lipidomics
Libre accès

Abstrait

Pharmacoproteomic of Aspergillus fumigatus for identification of novel molecular targets having application immuno diagnosis and therapy

Rambir Singh

Aspergillosis has emerged as threat to public health in recent past. Early stage diagnosis of aspergillosis has been difficult. There are limited options of effective drugs for treatment and invasive infections are always fatal. Importantly, clinical symptoms of aspergillosis overlap with those of TB. This often leads to misdiagnosis of aspergillosis as TB and wrong treatment. Hence, early diagnosis of aspergillosis is essentially needed. Currently, available late stage antigen based serological tests have limited diagnostic efficacy. Hence, there is urgent need for identification of novel molecules of diagnostic and therapeutic relevance. While working with Indian (ITCC-6604) and German (DAYA) strains of A. fumigatus, we were able to identify 111 cytosolic proteins spots from 2D gels. Out of these 111 protein spots, 66 proteins have been identified on comparison with available protein databases. Immuno-proteomics studies on these cytosolic proteins showed the presence of highly immunodominant IgG and IgE reactive proteins. Characterizations of these proteins have immense application in immuno-diagnosis and therapy of aspergillosis.