Amin Shiralizadeh Dezfuli1*, Hamed Afkhami2, Iman Menbari Oskouiec3, Leili Mohammadi4
We utilize a new nanocomposite material to give a report about a new platform for wound dressing. It is worth mentioning synergistic mixing graphene quantum dots (as a type of Organic Dots (ODs)) and Polyvinyl Alcohol (PVA) are materials participating in fabrication of the nanocomposite mentioned. We brought Staphylococcus aureus as a gram-positive bacterium and Pseudomonas aeruginosa as a gram-negative bacterium into play to optimize their antibacterial activity. We evaluated Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) for OD/PVA nanocomposite. Our optimizations resulted as a persistent level against Methicillin-Resistant Staphylococcus Aureus (MRSA) in higher ratios of OD. In-vitro studies and MTT assay were used for determination the optimum ratio of OD to PVA. We quantified burned wound infection models and the colony forming units CFUs in the burn wound which were in-vivo studies by a standard colony counting method. Finally, the results show the mechanism of antibacterial activity of nanocomposite described as a contact mediated oxidative stress induction.