Journal des maladies infectieuses et de la médecine préventive
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Occurrence of Staphylococcus aureus on Environmental Fomites in Ile Ife, Osun State

Joseph Omololu-Aso, A.Oyinloye, Oluwaseun O. Omololu-Aso, Oluwagbenro Adesunloro

Staphylococcus aureus, a Gram positive coccus has been recognized to be an important pathogen both in the Community and in the Hospital. The aim of this study was to determine the occurrence of Staphylococcus aureus on environmental fomites and to determine the antimicrobial susceptibility of the isolates recovered to various antibiotics. A total number of 50 samples were obtained from different sources in Obafemi Awolowo University. Ile-Ife. Osun State. These samples were collected from selected area of the University environment such as Banking area, Student’s hand mobile phones, door knobs, commercial bus driver’s steering and stair rails. The samples were analysed using standard conventional methods and materials in order to isolate, identify and carry out the antimicrobial susceptibility testing. Subsequent Culturing using nutrient broth was carried out. Nutrient agar, Mannitol Salt agar was used for the isolation process. Biochemical test such as Catalase test, Coagulase tests, DNase test, Antibiotic Sensitivity test were carried out to identify the isolates. Strict Sterilization process was ensured while working on the cultures and the materials used. The antibiotic susceptibility test was conducted using Kirby and Bauer disc diffusion method and the results was determined using the clinical and laboratory standard institute (CLSI) 2016 guide. The total number of 34 (68%) Staphylococcal isolates were recovered from 50 samples when streaked on Mannitol salt agar (MSA). And 19 (38%) isolates were confirmed to be Staphylococcus aureus. The highest rate of isolation of Staphylococcus aureus isolates were obtained from samples collected from student hand mobile phones, followed by samples collected ATM touch boards and commercial Bus steering. 100% of the Staphylococcus aureus isolates were susceptible to Streptomycin, followed by 78.95% of Ciprofloxacin and 73.68% septrin. All isolates were resistant to Amplicox and Amoxicilin. About 57.89% were susceptible to Zinnacef, 26.32% were susceptible to Gentamycin.