Immunothérapie : Libre accès
Libre accès

Abstrait

Neuroprotective Effect of Glial Cell-Derived Exosomes on Neurons

Sujin Hyung, Ju Young Kim, Chan Jong Yu, Hyun Suk Jung, Jong Wook Hong

Recent research has revealed that exosomes affect several aspects of organism physiology and development, as well as disease processes, through intracellular communication. However, the biological roles of exosomes in exosomesecreting cells have remained largely unexplored. In this study, we demonstrate that Schwann cell (SC)-derived exosomes (EXO^SC) promote cell survival among motor neurons (MNs), with MN viability exceeding 80% at days in vitro (DIV) 14. Prevention of MN cell death by EXO^SC was achieved by blocking the caspase-3 cell death pathway, which was confirmed by comparing the number of activated-caspase3+ cells according to the presence versus absence of EXO^SC. The attenuation of exosome secretion from SCs by treatment with GW4869 resulted in increased MN cell death, regardless of SC viability. Together, these findings enhance our understanding of exosome biology and provide valuable new insights into exosomes as a potential therapeutic agent in nervous system disorders.