Journal des techniques de chromatographie et de séparation

Journal des techniques de chromatographie et de séparation
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ISSN: 2157-7064

Abstrait

Isolation and Quantitative Estimation of Quercetin in Lagenaria siceraria Fruit

Sharada L Deore, Kumudini Nikole, Bhushan A Baviskar and Somshekhar S Khadabadi

Lagenaria siceraria is an important medicinal plant belonging to family Cucurbitaceae. Now a day its use in Juice form is very popular. In present article, quercetin has been isolated from ethyl acetate fraction of fruit extract and confirmed by Co-TLC, UV and FTIR analysis. In addition, a simple, rapid, precise, and accurate high-performance thin-layer chromatographic (HPTLC) method has been established, validated for flavonoid quercetin. The linearity of the method was investigated in the range of 1-3.5 ng mL-1. Percentage recoveries for quercetin found to be 99.79%. The method was validated for precision, accuracy, specificity, and ruggedness. Densitometric quantification was performed at 575 nm after spraying with aluminum chloride reagent.

Clause de non-responsabilité: Ce résumé a été traduit à l'aide d'outils d'intelligence artificielle et n'a pas encore été révisé ou vérifié.
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