Ingénierie enzymatique
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Abstrait

Finding Gene Mutations by the Enzyme: Chips for a Simple and Highly Sensitive Enzyme Mismatch Cleavage Method

Yo Niida

Determination of unknown DNA variations is one of the substantive matters in many fields of molecular biology. Sanger sequencing has been used to the routine for this purpose. However, when you need to examine a large sized DNA or abundant samples, this method is bothersome, expensive and time consuming. Recently, next generation sequencing (NGS) has been used in various purposes of mutation screening [1]. This massive sequencing technology is suitable for a scale of genome size screening, as well as to screen the list of genes that cause similar phenotypes, such as maturity onset diabetes of the young (MODY) [2]. If enough samples are gathered at once, NGS is a hopeful and fascinating strategy, because pooling of samples lowers the running cost per sample. But, if you are intended to examine 10~50 kb of DNA sequence by single experiment, you require an efficient and convenient screening method.