Journal de protéomique et bioinformatique
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Evaluation of rbcL Gene in African Yam Bean (Sphenostylis stenocarpa Hochst Ex. A Rich Harms) and Related Legumes Using in Silico Approach

A. N. Osuagwu, U. L. Edem, O. P. Bassey, E. C. Ije

Computation study of target genes has proven to be useful in contemporary research. This research was carried out to assess variation in rbcl gene of African yam bean (Spenostylis stenocarpa) and related legumes in the Fabaceae family. Sequences of rbcL gene were downloaded from National Center for Biotechnology Information (NCBI) database and used for this study. Multiple sequence alignment was carried out on the nucleotide sequences and all gaps were excluded from the analysis. The results of the phylogenetic analysis grouped the legumes into two major clades, while the maternal limeage analysis showed that African yam bean must have originated from Cajanus cajan (pigeon pea) and Glycine max (soy bean). The Guauine-cytosine content of the plants was similar and the domain detected in the sequences was low complexity which was the same in all the legumes into two major clades, while the maternal lineage analysis showed that African yam bean must have originated from Cajanus cajan (pigeon pea) and Glycine max (soy bean). The Guanine Cytosine (GC) content of the plants was similar and the domain detected in the sequences was low complexity which was the same in all the legumes sampled in this study. The present study suggests high similarly among the legumes based on information on their rbcl gene.