Journal de Fertilization: In Vitro - IVF-Worldwide, Médecine de la Reproduction, Génétique & Biologie des Cellules Souches
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Effect of Physico-Biochemical Characteristics of Follicles on Quality and In Vitro Maturation of Bubaline Oocytes

S Satheshkumar, B Revathi Priya, K Brindha, A Roy and K Kumanan

The physico-biochemical properties of follicles and its plausible effect on quality and maturational competency of oocytes during the different breeding periods of the year was studied in buffaloes (Bubalus bubalis). Ovaries were collected from sexually mature buffaloes slaughtered at abattoir during high breeding (December- February; HB) and low breeding (April-July; LB) periods of the year. The surface antral follicles were categorized as small (SF; < 4 mm), medium (MF; 4-9 mm) and large (LF; >9 mm) follicles. The follicular fluid (FF) was aspirated from follicles of different categories and the recovered oocytes were assessed for their quality. The good quality oocytes were cultured in vitro to assess the maturational competency. The glucose (Glu), total proteins (TP) and triglycerides (Tgl) concentrations of FF were analysed and correlated with the oocyte quality and maturation capacity. Among the follicular categories, significantly (P