Immunothérapie : Libre accès
Libre accès

Abstrait

Early Mixed Inflammatory Response in Children with Sepsis

Cen Li, Xia Lv, Xia Fan, Dong Huang*, Jianquan Li*

Objective: By dissecting systemic inflammatory response, we aimed to provide the reference to treatment for pediatric patients with sepsis.

Methods: 62 pediatric children with sepsis and 48 pediatric children without sepsis were enrolled in study. HLA- DR/CD14+ expression, proportion of CD4+CD25+ Forkhead box protein P (Foxp3+) Treg cells and IL-27+, CD4+ cells were analyzed by flow cytometry. Foxp3, CTLA-4, GITR, IL-10, L-17A, IL-17F and IL-27 mRNA levels were evaluated by real-time PCR. IL-4, IFN-γ and TGF-β were measured by enzyme-linked immunosorbent assay.

Results: Compared to children without sepsis, there are higher level of IFN-γ, IL-17A, IL-17F, IL-27 mRNA, Foxp3, CTLA-4, IL-10 mRNA and proportion of CD4+, IL-27+ cells and lower of TGF-β and CD4+CD25+Treg cells in children with sepsis. The HLA-DR was significantly lower in sepsis group, but greater than 30%.

Conclusion: Pro-inflammatory response dominates at early stage in sepsis children. Therefore, removing inflammatory response rather than immunomodulation is key of treatment for pediatric sepsis.