Journal des techniques de chromatographie et de séparation
Libre accès
ISSN: 2157-7064
ISSN: 2157-7064
Santini DA, Sutherland CA and Nicolau DP
A simple and reliable high performance liquid chromatography method was developed and validated to analyze tedizolid in human plasma, human serum, saline and CD-1 mouse plasma. An ultra violet detector set at 251nm was used with a reverse phase column. The mobile phase consisted of sodium acetate, deionized water and acetonitrile at a flow rate of 1.0 ml/min. 4-nitroaniline was used as the internal standard. The standard curves were linear over a range of 0.2 to 5 μg/ml. Accuracy and precision for the validations were within the acceptable 10% limit. This method was successfully used to assay samples previously analyzed by liquid chromatography with tandem mass spectrometric detection.