Journal des techniques de chromatographie et de séparation
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Development and Validation of TLC-densitometry Method for Simultaneous Estimation of Brimonidine tartrate and Timolol maleate in Bulk and Pharmaceutical Dosage Form

Pritam S. Jain, Rahul N. Khatal, Hardik N. Jivani and Sanjay J. Surana

A new simple , precise, accurate and selective TLC-densitometry method has been developed for simultaneous determination of brimonidine tartrate and timolol maleate in pharmaceutical formulation. Chromatographic separation was performed on aluminum plate precoated with silica gel RP-18 F 254 using methanol: water: triethylamine (2.0:2.0:0.2 v/v) as mobile phase. Detection was carried out densitometrically at 264 nm. The R f value of brimonidine tartrate and timolol maleate were 0.23 and 0.63, respectively. The reliability of the method was assessed by evaluation of linearity which was found to be 300 – 1800 ng/spot for brimonidine tartrate and 1000 - 6000 ng/spot for timolol maleate. Accuracy of the method was accessed by percentage recovery and found to be 99.77 ± 0.71 % for brimonidine tartrate and 99.87 ± 0.86 % for timolol maleate. The method can be used for routine analysis of brimonidine tartrate and timolol maleate in pharmaceutical formulation.