Journal des techniques de chromatographie et de séparation
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Cutting-edge Proteomics: Techniques and their Applications

Sravani Neelapu

Proteomics involves the application of technology to determine the content and size of the total protein content of a cell, tissue, or organism. It replaces other "omics" techniques such as genomics and transcriptomics to explain the identity of an organism's proteins and to identify the structure and functions of a particular protein. Proteomics is practically complex because it involves the analysis and classification of the total protein signatures of a gene. Mass spectrometry devices with LC-MS-MS and MALDI-TOF/TOF are widely used in current proteomics. However, the use of Proteomics facilities, including software for equipment, databases, and the need for skilled personnel, significantly increases costs, thus limiting their widespread use, especially in developing countries. Furthermore, the protein is highly dynamic due to the complex control systems that control the expression levels of the proteins. This review attempt to explain the various proteomics approaches, recent developments and their application in research and analysis