select ad.sno,ad.journal,ad.title,ad.author_names,ad.abstract,ad.abstractlink,j.j_name,vi.* from articles_data ad left join journals j on j.journal=ad.journal left join vol_issues vi on vi.issue_id_en=ad.issue_id where ad.sno_en='50197' and ad.lang_id='5' and j.lang_id='5' and vi.lang_id='5' Apolipoprotein B-100 Peptide p210 Inhibits Proliferation of | 50197
Journal d'immunologie clinique et cellulaire

Journal d'immunologie clinique et cellulaire
Libre accès

ISSN: 2155-9899

Abstrait

Apolipoprotein B-100 Peptide p210 Inhibits Proliferation of Naïve T Effector Cells and Promotes Induction of Tolerogenic Antigen Presenting Cells and Regulatory T Cells in Vitro

Sara Rattik, Caitriona Grönberg, Maria F Gomez, Harry Björkbacka, Gunilla Nordin Fredrikson, Jan Nilsson and Maria Wigren

Objectives: Modulation of immune responses against LDL antigens through therapeutic vaccines represents a possible new approach for prevention of cardiovascular disease. The mode of action of these vaccines remains to be fully characterized but the protective effect of immunization with the apolipoprotein B-100 (apoB-100) derived peptide p210 has in several studies been associated with activation of regulatory T cells. The present study used an in vitro model to study the effect of p210 on immune cells.
Methods and results: CD11c+ antigen presenting cells, CD25-CD4+ naïve T effector cells and CD25+CD4+ T regulatory cells were isolated from mouse spleens using antibody-coated magnetic beads. Pre-incubation of antigen presenting cells with p210 conjugated to cationized bovine serum albumin (p210-cBSA) down-regulated the expression of CD86 and MHC class II molecules, inhibited proliferation of pre-activated naïve T effector cells and stimulated conversion of these cells into regulatory T cells. These effects were shown to partly be mediated through a suppression of the release of IL-12 from antigen presenting cells.
Conclusions: The present findings demonstrate that p210-cBSA inhibits proliferation of naïve T effector cells and promotes their conversion into regulatory T cells and this is suggested to be associated with a reduced activation status of antigen presenting cells. Taken together these findings suggest that immunization with p210-based vaccines have the capability of inducing tolerogenic APCs that in turn generate regulatory T cells suppressing T effector cell functions.

Clause de non-responsabilité: Ce résumé a été traduit à l'aide d'outils d'intelligence artificielle et n'a pas encore été révisé ou vérifié.
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