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Antiscorpion Venom Activity of an Aromatic Compound having Carbohydrate Moiety Isolated from Hemidesmus indicus (Anantamul) Root Extract in Experimental Animal Models

Antony Gomes*, Rinku Das, Sourav Ghosh

The present study established the anti scorpion venom activity of an aromatic compound purified from the Indian medicinal plant Hemidesmus indicus (anantamul) root extract in experimental animals. The compound (H.I-1) was isolated by silica gel column chromatography and thin layer chromatography and spectral analysis was done by IR and H1 NMR. Animal ethical clearance was obtained before experiment. Anti scorpion venom activity was evaluated using various in vivo tests, urine qualitative analysis, renal toxicity markers (urea and creatinine), hepatotoxicity markers (AST and ALT), myotoxic markers (LDH and CPK) and oxidative stress markers (LPO, GPX and GSH) in animal models. Statistical analysis was done by one way ANOVA, values expressed as mean±SEM, P<0.05 was considered as statistically significant. Silica gel column chromatography I & II produced an active compound (H.I-1) which gave a single spot on TLC. IR and H1 NMR studies revealed that H.I-1 was aromatic compound having sugar (rhamnose) moiety. It could neutralize scorpion (Heterometrus bengalensis) venom induced hepatotoxicity, renal toxicity, myotoxicity and oxidative stress in experimental animals. These observations confirmed that the aromatic compound H.I-1, isolated from Hemidesmus indicus root extract might be useful in scorpion envenomation and warrants further detailed studies.