Journal d'ophtalmologie clinique et expérimentale
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Acellular Human Amniotic Membrane Scaffold Loaded with Nanoparticles Containing 15d-PGJ2: A New System Local Anti-Inflammatory Treatment of Eye Diseases

Julio C. Francisco, Rossana B. Simeoni, Ricardo C. Cunha, Marco A. Cardoso, Bassam F. Mogharbel, Luiz C. Guarita-Souza, Katherine A. T. de Carvalho, Marcelo Napimoga and Luiz F. Moreira Pinho

The pathogenesis of chronic inflammatory eye diseases is multifactorial and includes factors as tissue injuries, metabolic disorder and autoimmune diseases. The 15-deoxy-Δ12, 14-PG J2 is known for its anti-inflammatory, antioxidant and immunmodulatory properties. In vivo adhesions between cells and the extracellular matrix play a crucial role in cell differentiation, proliferation, and migration as well as tissue remodeling. Here, we present a simple method to incorporate 15d-PGJ2 nanoparticles in acellular human amniotic membrane (HAM) scaffold, as potential local anti-inflammatory delivery system. After completely removing the cells on the amniotic membrane with a sodium dodecyl sulphate and mechanical approach, we seeded Vero cells incorporate 15d-PGJ2 nanoparticles on it. The morphology of the Vero cells and nanoparticles was observed by scanning electron microscopy (SEM). The cells cultivated observed by scanning electron microscopy (SEM) presented the incorporation of the nanoparticles smooth surface and spherical shape. Our results indicate that the HAM may be an ideal candidate as a nanoparticule-matrix adhesion substrate to study a new system for local anti-inflammatory therapy.