Journal des techniques de chromatographie et de séparation
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A Simple and Modified Method Development of Vancomycin Using High Performance Liquid Chromatography

Meenakshi K Chauhan and Nidhi Bhatt

Vancomycin is well known as a prominent member of the glycopeptide class of antibiotics. In this paper, a rapid resolution method using high performance liquid chromatography is employed to identify the presence of Vancomycin. A robust method is established and validated for the simultaneous quantification of glycopeptides antibiotic like Vancomycin and applied for their pharmaceutical research. This method is simple, modified, user and environment friendly with new techniques and all sample preparations are performed in excellent manner. The method showed good sensitivity with 2 μg/mL limit of quantification (LOQ) and the calibration curve is linear in the range of 2-500 μg/mL concentration range. The within- run and between- run precision obtained is less than 2 and 4% respectively. The method is proposed to be applied in an experimental micro particulate carrier based oral study design. The results can proved to be specific, linear, accurate and sensitive and the available data can provide valuable information regarding the analysis and pharmacokinetics of Vancomycin after the oral administration.